Assessing opioid regulation of adenylyl cyclase activity in intact cells.

1 Molecular Cloning of Opioid Receptors by cDNA Library Screening Ying-Xian Pan ......................................................................................... 3 2 Expression of Opioid Receptors in Mammalian Cell Lines Ying-Xian Pan ....................................................................................... 17 3 Assessing Opioid Regulation of Adenylyl Cyclase Activity in Intact Cells Deepak R. Thakker, Hatice Z. Ozsoy, and Kelly M. Standifer ..................................................................... 29 4 Analysis of Opioid-Induced Kinase Activation Lan Ma ................................................................................................... 39 5 Study of Opioid Receptor Phosphorylation Using Cell-Labeling Method with P-Orthrophosphate Jia Bei Wang ......................................................................................... 47 6 Opioid Receptor Coupling to GIRK Channels: In Vitro Studies Using a Xenopus Oocyte Expression System and In Vivo Studies on Weaver Mutant Mice Kazutaka Ikeda, Mitsunobu Yoshii, Ichiro Sora, and Toru Kobayashi ........................................................................ 53 7 Identification of Alternatively Spliced Variants from Opioid Receptor Genes Ying-Xian Pan ....................................................................................... 65